| 1. | H - 13 ( achromobacter ) . can facilitate the metamorphosis of ascidian larvae
无色杆菌属的h - 13能诱导幼体变态。
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| 2. | Furthermore , l - thyroxine do n ' t show any effects on the settlement and metamorphosis of ascidian larvae . 3
此外， l - thyroxine对冠瘤海鞘幼体的附着变态均未表现出显著的促进或抑制作用。
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| 3. | We also found crude extracts of two sponges ( mycale sp . and renieera spi ) can inhibit the settlement and metamorphosis of ascidian larvae . the two extracts are all liposolube
Spl ） ，它们的粗提物对冠瘤海鞘幼体的附着和变态有抑制作用，这两种粗提物都是脂溶性的。
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| 4. | During experiment , acetylcholine shows significant inhibitory effects on the settlement and metamorphosis of ascidian larvae , but its concentration cannot lower 1 x 10 - 7mol / l . similarly acetylcholine , y - aminobutyric ( gaba ) and picrotoxin also can inhibit the settlement and metamorphosis of s . canopus larvae
实验中，乙酰胆碱对冠瘤海鞘的附着变态表现出抑制作用，但当浓度降低到10 ~ ( - 7 ) mol l时，此作用已不明显。
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| 5. | According to the frequently used methods of extraction and isolation of natural products from organic body , the freeze - dried ascidian tunica was exhaustively extracted with 100 % chloroform then the combined extracts were filtered and the solvent was separately concentrated under vacuum to yield a dark - brown gum
按照目前比较常用的天然产物的提取分离办法，将样品冷冻干燥后，用100氯仿浸泡，收集氯仿滤液，减压蒸馏浓缩去除浸提溶剂，得到黑褐色的浸膏状物质。
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| 6. | Considering of the specificity of the degenerative primer designed in this pcr reaction , the identity between the sequence we wanted and the fragment of pcr product and the presence of asmaspa , asmaspb , clr and cls ( the homologous gene of masp gene ) in halocynthia roretzi , a japanese ascidian , we believe that the sequence of pcr product is some part of the masp gene or masp homologous gene
基于本实验中所设计的引物为特异性简并引物，测序基因通过比较得到与预期片段有一定的同源性以及masp同源物asmaspa 、 asmaspb 、 clr和cls在海鞘中存在的事实，我们可以初步推测，本实验pcr反应所克隆的片段可能为文昌鱼masp或其同源基因的一部分序列。
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