Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and 150mg / l kanamycin and incubated at 25 , 16 / 8h light / dark cycle . leaves of adventitious shoots were detected by gus staining Sin旮16ea ）上暗培养3天后，移入加有抗生素150mg ikan和500mg几cr的诱芽培养基上诱导不定芽的分化。
2.
Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25 , 16 / 8h light / dark cycle . small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution . positive shoots , gus tinted , were induced to root 分化出的不定芽切下半张叶片进行gus染色，呈阳性的植株从外植体上切下，在生根培养基（ ms 0刀sing lnaa 50mg几kan ）中诱导根的形成。
3.
The results also revealed that it was a reasonable choice to use carbenicillin as the antibiotics of inhibiting growth of remnant agrobacterium after co - culture , and when hygromycin was used as the selective agent , continuous selection at low concentrations ( 50 ~ 150mg / l ) produced the highest numbers of transgenic plants without escapes 试验表明，在农杆菌介导高羊茅遗传转化中，抑菌剂宜选用梭节青霉素;以潮霉素作为选择剂时，采用低浓度( 50一15om留l )连续筛选的方式比较合适，在该方式下，获得的转基因植株较多。
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