An endoenzyme, or intracellular enzyme, is an enzyme that functions within the cell in which it was produced. Because the majority of enzymes fall within this category, the term is used primarily to differentiate a specific enzyme from an exoenzyme.
例句与用法
1.
Constructing human colorectal cancer cell line with stably down - regulated grp94 ( 1 ) the plasmid prc / rsv - ribol that contains specific grp94 - targeting ribozyme and the control plasmid prc / rsv were miniprepared , respectively , cleaved by endoenzyme pvuii 稳定下调grp94的人大肠癌细胞克隆株的构建( 1 )分别对含有特异性打靶grp94核酶的质粒prc rsv - ribo1和对照组质粒prc rsv进行小量提取、 pvu酶切鉴定。
2.
This time , using cdna of zmcdc5 as template , we amplify a sequence by means of pcr technology . and then , using restrict endoenzyme and ligase , we conjunct the 0 . 8kb length dna sequence in a expression vector , pet - 30a . after induction , expression and purification , we obtained a 35 . 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ) . with the purified protein , we got its antibody and testified the antibody by means of western blotting and dot blotting 本实验是以zmcdc5的cdna为模板，使用pcr获得基因片段，再通过酶切连接，将得到的0 . 8kb的基因片段构建于pet - 30a表达载体上，经过诱导表达和纯化，获得zmcdc5的融合蛋白，其中包括了zmcdc5925个氨基酸中647 914共267个氨基酸残基
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