The western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22kd and 55kd proteins , which were existed in the testis tissue protein of mouse , rat and human . 2 ) the purified peptide emulsified in an equal volume of freund ' s adjuvant and immunized the female balb / c mice with 8 - 10 weeks old . the antiserums and the washings of vaginal membrane were detected by elisa , and shown the highest level of the specific antibody igg was 1 : 6000 , while the iga was 1 : 300 二、 p3多肽与弗氏佐剂混悬后免疫近交系balb c小鼠后，在血清和阴道粘膜冲洗液中可检测出特异性lgg 、 lga ，最高效价分别达到1 ： 6000和1 ： 300 ；免疫后的小鼠脾脏淋巴细胞增殖率升高，淋巴细胞培养上清液中分泌的il 4和inf y也升高，且以il 4更明显。
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