| 1. | While cortical alveoli discharge in batches , the plasma membrane has been reorganized time after time . spermaster " elongate and traction , and cytoplasm " flowage make the male pronucleus and the female pronucleus conjugate
）十物’ i ：他地仰排原核纪合是精人己光扩怅、引和细w质流动儿d 。
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| 2. | These results indicate that chx at an app ropriate concentration can improve the parthenogenetic development of buffalo oocytes , cb at an appropriate concentration may inhibit the extrusion of the first polar body and increase the proportion of activated oocytes with two pronuclei
结果表明，适当浓度的chx能提高卵母细胞激活效果，适当浓度的cb可抑制卵母细胞第二极体的排出，提高孤雌激活后双原核的比例。 2
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| 3. | On the other hand , - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established . methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation . this vector is used to pronucleus microinjection
本实验以pegfp - n1质粒为骨架载体，用酶切连接的方法构建一个顺序含有- actin启动子、 fad2cdna 、 sv40polya加尾信号的真核表达载体，双切线性化后回收，使用回收的表达载体经原核显微注射生产转基因小鼠。
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| 4. | The mice showed also ageing - associated decline in oocyte quality , as indicated by increased frequency of premature chromatid separation , spindle disruption and / or chromosome misalignments in mii oocytes of aged mice . the gv oocytes from aged mice normally matured in vitro . the percentages of pronuclear - stage embryos , 2 - cell embryos and blastocyst derived from mii oocytes of aged mice by in vitro fertilization ( ivf ) were comparable with ones of young mice , but pronuclei formed 1 h later in aged mice than in young mice
老龄小鼠gv期卵母细胞能正常体外成熟；老龄小鼠m期卵母细胞经体外受精后形成原核期胚、 2 -细胞期胚和囊胚的比率与年轻小鼠比较无显著差异，但原核形成时间延迟1小时；在老龄小鼠卵母细胞，注射牛精子抽提物或sr ~ ( 2 + )处理时所诱发的ca ~ ( 2 + )振荡以及在减数分裂成熟及早期胚胎中的pkc分布未发生改变。
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| 5. | Using the hprt gene as a positive control , our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist . female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too . while since the 4 - cells period , xist is constantly transcribed until blastocyst stages
然后利用实验一确定的pcr条件，以hprt为阳性对照，用巢式rt - pcr对小鼠早期胚胎进行xist基因的转录分析，结果发现，转录sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录xist基因；不转录sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2 -细胞期的过程中， xist基因一直不转录，但是，从4 -细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录xist基因。
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| 6. | The percentage of oocytes cleaved and formed pronucleus in either 0 . 625 ( ag / ml or 1 . 25ug / ml chx group was significantly higher than the control group ( p < 0 . 05 ) . however , when the concentration of chx was increased to 5 ug / ml , the cleavage rate and pronuclear formation rate were decreased significantly ( p < 0 . 01 )
结果培养在0 . 625和1 . 25 g mlchx的卵母细胞的激活分裂率和总原核形成率明显高于对照组( p 0 . 05 ) ，而培养在5 g mlchx的卵母细胞的激活分裂率和原核形成率则极显著低于对照组( p 0 . 01 ) 。
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