| 1. | Private treaty grant ptg
私人协约方式批地
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| 2. | Ptg private treaty grant
私人协约方式批地
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| 3. | 5 . after induced with iptg , jm109 with pgex - 4t - l / 6 - 4 was radiated by uv for 30 seconds , cultivate these e . coli with light or without light . the survival rate proved the gene of d . salina ( 6 - 4 ) photolyase has photoreactivation
5 .工ptg诱导过的转化菌，经过30秒的紫外光照射后，分别进行光培养或暗培养，计算存活率，从而证明了及sa了z ’ na的( 6一4 )光裂合酶在大肠杆菌中可进行光修复活性作用。
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| 4. | The e . coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4 . 4 . the transformation was induced with iptg , then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein , and the fusion protein is about 90kd
4 .用工ptg诱导含pgex一4t一1 / 6一4的转化菌，提取初提物中的总蛋白，进行sds一聚丙烯酞胺凝胶电泳，检测表达的融合蛋白大小越为gokd 。
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| 5. | Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised , and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst
Ptg诱导表达，超声破碎细胞后，采用亲和层析方法纯化融合蛋白gst十nadc3 ，并以此为抗原免疫新西兰株白兔制备融合蛋白抗体。应用亲和层析的方法对gst十nadc3融合蛋白抗体进行纯化，以去除抗gst抗体。
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| 6. | Its content was about 9 . 8 % among total cell protein by gene genius bio imaging system . the fusion proteins were found largely in an insoluble inclusion bodies . the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105
经工ptg诱导，重组质粒在点co力‘ blzi中表达出了c端融合了6xhis的融合蛋白，过量表达的蛋白主要以不溶性蛋白形式存在，其表达量占菌体总蛋白的9 . 8 % 。
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