| 1. | Now the serpent was more subtil than any beast of the field which the lord god had made
耶和华神所造的，惟有蛇比田野一切的活物更狡猾。
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| 2. | And , behold , there met him a woman with the attire of an harlot , and subtil of heart
看哪，有一个妇人来迎接他，是妓女的打扮，有诡诈的心思。
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| 3. | [ kjv ] and , behold , there met him a woman with the attire of an harlot , and subtil of heart
看哪，有一个妇人来迎接他，是妓女的打扮，有诡诈的心思。
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| 4. | Gen 3 : 1 now the serpent was more subtil than any beast of the field which the lord god had made
创3 : 1耶和华神所造的，惟有蛇比田野一切的活物更狡猾。
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| 5. | Pro 7 : 10 and , behold , there met him a woman with the attire of an harlot , and subtil of heart
箴7 : 10看哪，有一个妇人来迎接他，是妓女的打扮，有诡诈的心思。
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| 6. | But amnon had a friend ， whose name was jonadab ， the son of shimeah david ' s brother ： and jonadab was a very subtil man
3暗嫩有一个朋友，名叫约拿达，是大卫长兄示米亚的儿子。这约拿达为人极其狡猾。
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| 7. | Sequencing analysis showed that the sequence of endoglucanase fragment exhibits 35 % homology with b . subtil is chromosomal dna ( from glyb to apre ) , and 27 % homology with bacillus sp
将此重组质粒phchi转化巨大芽抱杆菌b . megateriumap25 ，得到两株转化子，分别命名为p25113一9 ， p25113一10 。
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| 8. | By transformation with the genes . plant disease biocontrol bacteria bacillus subtil is aplls and b . megaterium ap25 were isolated from wheat field soils collected from south australia and tai an . enzyme activity analysis on chitin agar and abp media showed that b . subtilis aplls secreted chitinase and b . megaterium ap25 secreted endoglucanase , respectively
测序后在genebank上进行序列比较，该基因片段同编号为2634966的枯草芽孢杆菌全序列的2599451到2812870 (功能未知)有85的同源性，但同已发表的13种几丁质酶的基因(包括枯草芽孢杆菌几丁质酶基因)的同源性很低，只有30 。
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| 9. | In order to express alkaline protease gene ( ap gene ) in bacillus subtil is , the recombinant expression plasmid was constructed . this plasmid contains a promoter bp53 , also from b . pumilus un - 31 - c - 42 , ap gene and the shuttle vector psugv4 . after introduced into b . subtilis wb600 , the transformants displayed the hydrolyzed zone on milk plate
将来自短小芽抱杆菌un一31一c礴2的基因启动子( bp53片段)和脱毛蛋白酶全基因( ap )进行融合，然后将重组基因(命名为bpap )插入到大肠杆菌-枯草杆菌穿梭质粒载体psugv4中，构建成表达质粒psu一bpap 。
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