Synthesis and applications of 2 - phenylmethyl thio - 3 - pydine - carboxylic acid 吡啶甲酸的合成及应用
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Thio - urea dioxide manufacturer -生产各类氟化物。
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The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others , especially by lixinlal in naigin normal university of china , we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence , using the favorable condon of the ecoli . after cheked the result of synthesis by sequence , we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ) , and the fusion protein of thio - cmiv was highly expressed in soluble form 本研究的内容包括：一、在前人对抗菌肽cmiv研究的基础上，对n端和c端进行氨基酸保守变换，设计和合成了该基因，充分使用大肠杆菌偏爱的密码子，并将该基因5端与硫氧还蛋白基因3端融合，通过ptxfus表达载体获得较高可溶性表达(在15 sds - page胶上可见明显的表达蛋白带) 。
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